rabbit anti aco2  (Proteintech)

Journal: The Journal of experimental biology

Article Title: Effects of actin remodeling inhibitors on cellular energy metabolism of a model marine bivalve, the Pacific oyster.

doi: 10.1242/jeb.249708

Figure Lengend Snippet: Fig. 5. Immunoblot analysis and quantification of the actin-to-aconitase ratio in mitochondria isolated from C. gigas gill cells incubated with either DMSO (vehicle control) or cytochalasin D. (A,B) Immunoblot analysis of protein levels of aconitase (A) and actin (B). Negative sample numbers represent the vehicle control, while positive numbers correspond to cytochalasin D-treated cells from the same isolate. L.c., loading control. (C) Quantification of the actin-to-aconitase ratio in mitochondria isolated from C. gigas gill cells incubated with either DMSO (vehicle control) or cytochalasin D. No significant differences in the actin-to-aconitase ratio were observed between control and cytochalasin D-treated cells (ratio paired t-test, P>0.05). N=6.

Article Snippet: Lysis was conducted on ice for 2 h. Proteins were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride (PVDF) membrane (Merck KGaA, Darmstadt, Germany) and probed with either anti-aconitase antibody (Cell Signaling, cat. no. 6571) or anti-actin antibody (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, cat. no. A2066).

Techniques: Western Blot, Isolation, Incubation, Control

Journal: Biochemical and biophysical research communications

Article Title: Low expression of Frataxin might contribute to diabetic peripheral neuropathy in a mouse model.

doi: 10.1016/j.bbrc.2024.151228

Figure Lengend Snippet: Fig. 4. SS-31 improves mitochondrial function and ameliorates inflammation in sciatic nerves of db/db mice by elevating the expression of Fxn. β-actin is used as an internal control if not specified in this figure and later. (A, B) SS-31 increases the expression of Fxn, revealed by immunoblotting, in SCN of SS-31-treated db/db mice. n = 6. (C-E) Expression of Ndufs1 and SdhB proteins in SCN of SS-31-treated db/db mice. n = 6. (F, G) Fe–S dependent aconitase activity in SCN. n = 6. Middle panel showed the protein levels of Aco2. (H) The activities of mitochondrial respiratory complex I (NADH-ubiquinone oxidoreductase), determined using spectrophotometry. n = 3. (I–K) qRT-PCR analysis of sciatic nerve mRNA levels of pro-inflammatory cytokines (Il-6, Tnf-α, and Il-1β) after SS-31 treatment. n = 3. Data are mean ± SD. kDa: molecular weight in kilodalton. ANOVA was used for significance. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The primary antibodies used are as follows: anti-Ndufs1 (Proteintech, catalog number 12444-1-AP), anti-Rabbit Aco2 (Proteintech, cat# 11,134–1-AP), anti-Ho-1 (Cell Signaling Technology, catalog number 70081 S), anti-Ftl (Abcam, cat# 69,090), anti-SDHB (Abcam, catalog number 178423), and polyclonal antibodies against Fxn and FtH (homemade, prepared from rabbits).

Techniques: Expressing, Control, Western Blot, Activity Assay, Spectrophotometry, Quantitative RT-PCR, Molecular Weight